| Carrollton School of the Sacred Heart | Carrollton School | A | 8 | Lauren Russler '22 * | 1-12 | Natalie Scherr '20 | 1-12 |
| B | 20 | Natalia Freyre '23 | 1-12 | Sarah Gutierrez '23 * | 1-12 |
| Dunedin High School | Developing All Stars | A | 16 | | | | |
| B | 24 | | | | |
| Fort Myers High School | Green Wave | A | 20 | Bo Dwyer '22 | 1-12 | Morgan Gahan '22 | 1-12 |
| B | 14 | Zachary Syska '22 | 1-12 | Caroline Cicotte '21 | 1-12 |
| Gulliver Preparatory School | Raiders | A | 4 | Daniel Hughes '21 | 1-12 | Abigail Sharp '22 | 1-12 |
| B | 7 | Cort Snyder '23 | 1-12 | Karen Liberman '20 | 1-12 |
| H. B. Plant High School | Panthers | A | 19 | Garrett Brooks '22 | | Emmy DiGiacomo '20 | 1-8 |
| | Noelle Dennis '22 | 9-12 |
| B | 12 | Reid Nelson '23 | | Kate Prest '20 | 1-8 |
| | Spencer Brooks '20 | 9-12 |
| Jensen Beach High School | Falcons | A | 14 | Chloe Hudgins '20 | | Griffin Barron '21 | |
| B | 15 | Coby Flannery '21 | | Dina Coletti '20 | |
| MOA/Westwood High School | Panthers | A | 22 | Heriberto Lara-Mijares '20 | | Colin Flink '20 | |
| B | 21 | Nicholas Stange '20 | | Grace Lamb '20 | 1-8 |
| | Austin Daigle '20 | 9-12 |
| Martin County High School | Tigers | A | 18 | Marc Kiener '20 | | Cameron Lott '20 | |
| B | 18 | Kathryn Thorsen '21 | | Diego Cruz '23 | |
| Naples High School | Varsity | A | 13 | Lauren Connell '22 | | Maia Musial '22 | |
| B | 8 | Thomas Connell '20 | | Cooper Atkins '23 | 1-8 |
| | Conner Bricco '22 | 9-12 |
| Oxbridge Academy | ThunderWolves | A | 15 | Hilton Kamps '22 | 1-11 | Raquel Shulman '22 | 1-11 |
| B | 19 | Will Dimaio '22 | 1-11 | Aidan Deitrick '20 | 1-11 |
| Palm Harbor University High School | Varsity | A | 23 | | | | |
| B | 10 | | | | |
| Pine Crest School | Panthers | A | 17 | Bella Casaretto '22 | 1-12 | Rachel Becker '23 | 1-12 |
| B | 9 | Ryan Kronrad '22 | 1-12 | Thomas Yevoli '23 | 1-12 |
| Pine View School | Python Varsity | A | 12 | Dlyan Lopez '27 | | Mia Quijano '26 | |
| B | 16 | James McGirr '20 | | Dlyan Lopez '27 | |
| Ransom Everglades School | Raiders | A | 3 | Lachlain Mc Granahan '20 | 1-2,9-12 | Hannah Bast '21 | 1-2,9-12 |
| Stephan Baker '22 | 3-8 | Adam Kaplan '21 | 3-8 |
| B | 1 | Liam O'Keefe '21 | 1-2,5-8 | Claire Holzman '21 | 1-4,9-12 |
| Javier Garcon '21 | 3-4,9-12 | Nicolas Echarte '21 | 5-8 |
| Sarasota High School | Sailors | A | 10 | Matthew King '20 | 1-4 | Nick Powers '21 | 1-12 |
| Thommi Gritt '22 | 5-12 | | |
| B | 2 | Will Murray '20 | 1-12 | Kay Brumsvold '21 | 1-12 |
| Shorecrest Preparatory School | Chargers | A | 1 | Guthrie Braun '22 | | Thomas Cura '21 | |
| B | 4 | Heather Kerns '20 | | Zuri Patel '20 | |
| South Fork High School | SF BULLDOGS | A | 7 | Emily Bornarth '20 | | Ryan Ince '21 | |
| B | 13 | Katelyn Herter '20 | | Zuzanna Barska '22 | 1-8 |
| | Siena Pollis '22 | 9-12 |
| St. Petersburg High School | Green Devils | A | 6 | Emma Shakespeare '20 | | Hannah Marsicano '20 | |
| B | 5 | Emily Allen '21 | | Colin Funsch '20 | |
| St. Stephen's Episcopal School | Varsity | A | 9 | J.J. (Jessica) Smith '20 | 1-12 | Brandon Shirmer '23 | 1-12 |
| B | 11 | Kaitlyn Liebel '22 | 1-12 | Ben Defelice '23 | 1-12 |
| St. Thomas Aquinas High School | Raiders | A | 2 | Connor Boland '21 | 1-4,9-12 | Daniel Neville '21 | 1-8 |
| Robert (Bobby) Rielly '21 | 5-8 | Sam Juskiewicz '20 | 9-12 |
| B | 3 | Joey Meagher '20 | 1-4,9-12 | Kenna Reed '20 | 1-4,9-12 |
| Libby Redmond '20 | 5-8 | Isa DuPlessis '20 | 5-8 |
| The Pine School | Knights | A | 5 | Peter Foley '22 | | Jack Squier '21 | |
| B | 22 | Anthony Saia '20 | | Sara Velcani '21 | |
| Venice High School (FL) | Varsity | A | 24 | Kane Fenton '23 | 1-8 | Nick Waite '23 | 1-8 |
| Peyton Dec '23 | 9-12 | Eric VanGennip '20 | 9-12 |
| B | 23 | Zoe Watson '22 | 1-12 | Annabelle Riggs '20 | 1-12 |
| Vero Beach High School | Vero Beach | A | 21 | Nita Holloway '20 | 1-12 | Katherine Bounassi '23 | 1-12 |
| B | 17 | Sydney Ohs '23 | 1-12 | Catherine Lovelady '23 | 1-12 |
| Reserves | Sarah Eliason '21Johnmark Gaponiuk '20 |
| Westminster Christian School | Warriors | A | 11 | Enzo Menditto '22 | 1-12 | Joshua Pike '22 | 1-12 |
| B | 6 | Charlie Leigh '22 | 1-12 | Fernando Sierra '22 | 1-12 |
| Reserves | Mariana Sierra '23 |
